A recent study published in Molecular Metabolism (Batrow et al., 2025) sheds new light on the molecular mechanisms regulating lipid metabolism in brown adipose tissue (BAT). Using a conditional knockout mouse model with specific PPARα deletion in brown adipocytes (PPARαBATKO), researchers uncovered a previously unrecognized regulatory crosstalk between PPARα and ChREBPβ-driven de novo lipogenesis, with implications for our understanding of obesity-related metabolic disorders.
Among the tools used in this work, the Adiponectin (mouse) ELISA (#A05187) and Leptin (mouse/rat) ELISA (#A05176), both from Bertin Bioreagent, enabled precise quantification of circulating adipokines across experimental groups, providing key readouts to characterize the metabolic phenotype of knockout animals under standard chow and high-fat diet conditions.
This publication illustrates how validated immunoassay solutions from Bertin Bioreagent support fundamental research into metabolic diseases, from adipose tissue biology to obesity models.
The full application note, including protocol, results and reference, is available on the Bertin Technologies Application Center.
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