Context
Yeast cell lysis improvement for high quality intracellular content extarction
Yeasts have long been used in the agri-food industry, particularly in alcoholic fermentation and bread-making processes. Today, thanks to advances in genetics and metabolic engineering, their applications in biotechnology have expanded and diversified considerably, making them essential organisms for the production of compounds of interest.
However, due to the thickness and complexity of the yeast cell wall, extracting intracellular metabolites remains challenging. Yet access to these metabolites is crucial to improve our understanding of the signaling and regulatory pathways within these organisms.
In this application note, we present the results of yeast cell disruption obtained with the Precellys Evolution Touch, comparing the performance of a single bead size with that of a bead size mixture. In addition, an organic solvent mixture was tested to halt metabolism and facilitate access to intracellular content. Process efficiency was assessed by determining the percentage of cell lysis and evaluating membrane integrity using flow cytometry.
