Context
The kidneys are a pair of bean-shaped organs positioned on each side of the body. They are vital for maintaining homeostasis by filtering the bloodstream, eliminating waste products, and generating urine. Blood flows into the kidneys via the renal arteries and exits through the renal veins. Each kidney is linked to a ureter, which carries urine to the bladder.
Autosomal dominant polycystic kidney disease (ADPKD) is a widespread inherited condition, most commonly resulting from mutations in the genes that produce polycystin‑1 (PC1) or polycystin‑2 (PC2). The disease is marked by the development of numerous fluid-filled cysts within the kidneys, which can lead to complications including high blood pressure, kidney stones, repeated urinary tract infections, and eventually kidney failure. ADPKD affects around 12 million individuals globally. There is currently no cure, and cyst growth cannot be prevented.
The Bertin Precellys Evolution Touch homogenizer enables the extraction of RNA from multiple tissue samples while minimizing batch effects. In this study, it was specifically used to obtain high-quality RNA for RNA sequencing from both wild-type and cystic mouse kidneys.
This application note outlines the procedure for isolating high-quality RNA from mouse kidney tissue.
