Context
Precellys Evolution homogenizer can be integrated into a bacterial quantification workflow for contaminated water.
Bathing water, such as sea, lake, and river, may be subject to microbiological pollution. Enterococcus spp and E. coli are used as water-quality indicators in the European Union (Directive 2006/7/EC) and the United States (2012 Recreational Water Quality Criteria – RWQC published by U.S. Environmental Protection Agency (EPA)). In this work, we propose a multiparametric detection method that uses the Precellys Evolution* of these 2 microorganisms in less than 3 hours after bathing water collection by EU and EPA regulations. This rapid and quantitative analysis is based on RT-PCR, which is specifically dedicated to viable bacteria.