Context
Traditionally, in order to release intracellular retained rAAV vectors, producer cells are lysed by 3 to 4 freeze-thaw-cycles (liquid nitrogen-37 °C water), producing a crude cell lysate that is the starting material for a variety of subsequent purification and concentration methods. Therefore, efficient cell lysis is of crucial importance towards high-titer rAAV vector preparations. This application note investigates the use of the Minilys as an alternative solution to traditional freeze-thaw methods.
