[PRECELLYS] Drug extraction from brain, muscle rat tissues

Sources: PharmOptima, Portage MI, USA

Context

PharmOptima focuses on in vivo and in vitro DM&PK and Discovery Services, providing IND & NDAenabling research protocols. High quality DM&PK and Ocular PK research are a part of the laboratory’s daily activities. PharmOptima lab currently uses Precellys to homogenize a variety of animal tissues. In this study, Minilys has been compared with Precellys to homogenize brain and rat muscle.

Materials

Minilysvs Precellys Dual homogenizer
Precellys kit: 03961-1-009 (mix ceramic beads).
Sample: Thawed brain (~450mg) and muscle (~200mg).
Buffer: 2 volumes of sodium chloride solution.

Protocol

Minilys/Precellys: 5000 rpm, 2x45sec (15sec break).
Muscle tissue (triplicate):
Minilys: 5000 rpm, 3x40sec (15sec break).
Precellys: 5500 rpm, 3x30sec (15sec break).
Analysis: liquid-liquid extraction; LC-MS/MS system.

Results

The analyte levels by samples are equivalent. No difference involving the both homogenizers is detectable (See Figure 1).

Figure 1: LC-MS/MS analysis from Precellys and Minilys sample.

Figure 1: LC-MS/MS analysis from Precellys andMinilys sample.

Muscle tissues in CKmix kit 2mL vial

Minilys processing

Conclusion

Precellys and Minilys are suitable and reliable systems to extract Drug from soft tissues for DM&PK testing with a fast process, cross-contamination free, high reproducibility and comparable drug levels extracted. Minilys homogenizer is a real alternative for standardization of the sample preparationfor laboratories with a low or medium throughput of sample.