[PRECELLYS] Metabolite extraction from the red algae Chondrus crispus

The goal of this study was to establish the best protocol of extraction of the whole metabolome of algae. Seaweeds contain a lot of polysaccharides. For instance, in red algae, carrageenans confer a gelling nature to tissue, resulting in difficulties to obtain a reproducible manual grinding. Thus, particular attention has to be paid to grinding and automated techniques to carry out such extraction steps, as cryo-grinding, should be preferred. To avoid metabolite degradation, freeze-drying the sample before grinding is important. Thus, we tested automated cryo-grinding for the metabolite extraction of a red algae, Chondrus crispus, submitted to elicitation by methyl jasmonate (MeJA).