![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 49829](https://www.bertin-technologies.com/wp-content/uploads/2022/08/Group-56.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 49831](https://www.bertin-technologies.com/wp-content/uploads/2022/08/Group-57.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 51209](https://www.bertin-technologies.com/wp-content/uploads/2022/09/Group-cbrn-1.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 49833](https://www.bertin-technologies.com/wp-content/uploads/2022/08/Group-58.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 49835](https://www.bertin-technologies.com/wp-content/uploads/2022/08/Group-59.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 51211](https://www.bertin-technologies.com/wp-content/uploads/2022/09/Group-space-big-science-1.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 51200](https://www.bertin-technologies.com/wp-content/uploads/2022/09/Group-optronics.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 51204](https://www.bertin-technologies.com/wp-content/uploads/2022/09/Group-optomechanical.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 51202](https://www.bertin-technologies.com/wp-content/uploads/2022/09/Group-photonics.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 51212](https://www.bertin-technologies.com/wp-content/uploads/2022/09/Group-application-center.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 51214](https://www.bertin-technologies.com/wp-content/uploads/2022/09/Group-distributor-map.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 51216](https://www.bertin-technologies.com/wp-content/uploads/2022/09/Group-register-1.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 51218](https://www.bertin-technologies.com/wp-content/uploads/2022/09/Group-media.svg)
![[PRECELLYS] Polysomes immunoprecipitation and isolation of translating RNA from specific cell types of mouse brain samples Bertin Technologies 51220](https://www.bertin-technologies.com/wp-content/uploads/2022/09/Group-faq.svg)
Context
Precellys®24 homogenizer has been tested to perform polysomes immunoprecipitation (IP) and translating RNA extraction from IP. In our system, polysomes of some specific cell types of the cerebellum are immunoprecipitated, by expressing a GFP tagged ribosomal protein in these specific cell types with CRE mediated recombination. As these cell types represent a few percent of the whole cerebellum, we should observe an enrichment of specific genes in the IP fraction compared to the input fraction (whole cerebellum lysate before IP).
