RNA Extraction from normal dog skin biopsies - Bertin Technologies

RNA Extraction from normal dog skin biopsies

Dr Nicky Craig
Sources: College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA


Skin is very fibrous and elastic, and efficient RNA extraction from this tissue can be very difficult. We have previously tested several homogenizers to homogenize dog skin samples, with unsatisfactory results, including poor homogenization, poor RNA yield, and warming of the samples. To find a better option for RNA extraction from dog skin samples, several types of beads were tested for efficacy in homogenizing frozen skin biopsies using a Precellys® 24 Touch bead homogenizer.


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