[White paper] Best practices for tough sample grinding in molecular biology using Precellys

The biggest challenge when working with fibrous, elastic, and tough samples is efficient sample processing. The presence of elastic tissue, calcium phosphate (a rock-hard mineral), and other minerals can develop into a solid and tough matrix which can sometimes be difficult to homogenize.

Moreover, heat generation inside the lysing kit tubes during the process results in the degradation and denaturation of these molecules such as proteins and RNA. Heat generation is even more severe when working with harder samples that require higher energy for efficient homogenization, resulting in a more challenging process.

The Precellys® Evolution homogenizer can grind hard samples within minutes thanks to its 3D-bead beating technology. When paired with the Cryolys Evolution cooling unit, it also prevents heat generation inside the lysing kit tubes during the process, thus avoiding degradation and denaturation of thermo-sensitive molecules.