[PRECELLYS] Protein extraction from demineralized bones with Precellys Evolution

Bone Morphogenetic Protein 2 (BMP-2) is one of the most potent growth factors involved in bone growth and repair. Since it is present at low levels, its extraction and quantitation from bone is challenging. To evaluate the quantitative recovery of recombinant BMP-2 after a stringent grinding cycle in a Precellys® Evolution, we spiked commercial, demineralized cancellous bone with the protein, pulverized the samples, and measured the level of BMP-2 in the ground sample with an industry standard immunoassay.