[PRECELLYS] Comparative study of the use of the two-syringe method and the precellys emulsion kit

High quality emulsions are key for the success of the experiments in the research field of autoimmune diseases, among many others. However, the most commonly used method for creating emulsions today (two-syringe method), has two important limitations: 1) reproducibility, with high variability in the quality of the emulsions obtained between users, and 2) cost-efficiency, with a time-consuming procedure that also wastes a large quantity of costly reagents. The Precellys emulsion kit has thus been developed to overcome these limitations, and ensure the production of high-quality emulsions consistently.

The collagen-induced arthritis (CIA) mouse model is the most commonly studied autoimmune model of rheumatoid arthritis (RA). CIA can be induced in susceptible mouse strains by immunization with an emulsion of Freund’s adjuvant and type II collagen (CII). The onset of arthritis usually takes 3 to 5 weeks, and the incidence of the disease typically varies from 50% to 100%, depending on the mouse strain and the CII type.

This Application Note, presents the results obtained by researchers of Sorbonne Paris Nord University (INSERM U1125 Laboratoire Physiopathologie, cibles et thérapies de la polyarthrite rhumatoïde), in which a comparison between the quality of the emulsions created by the conventional two-syringe method and the Precellys emulsion kit is made. The evaluation is based on the onset, incidence and clinical scores of CIA for two different mouse strains.