[PRECELLYS] Drug extraction in rat heart samples

Drug concentration in tissues and body fluids change between the death and the postmortem specimen collections because of post-mortem redistribution. The aim of this study was to investigate post-mortem redistribution of the 2 cardiotoxic antipsychotic drugs: haloperidol and thioridazine, in order to interpret the postmortem redistribution. The rat has been chosen as the animal model.

See also the publication in the journal of Analytical Toxicology, 30(7)/ 419-25, September 2006, Nadege Castaing, KarineTitier, Mireille Canal-Rafin, Nicholas Moore,Mathieu Molimard.

• Precellys®24.
• Precellys® kit CK14 (small ceramic beads).
• Sample : rat heart (1/2 v/v).
• Buffer : water.

• Precellys®24parameters
• 6500 rpm, 2×15 sec.

Those chromatograms present liquid-liquid extraction results obtained from heart samples 6 hours after death. At this time the concentration of haloperidol and thioridazine was respectively 472 ng/g and 1435 ng/g (significant variation).

Chromatograms of Liquid/ liquid extraction.