[PRECELLYS] RNA extraction for bacteriological contamination control of sea, lake and river water

Bathing water, such as sea, lake and river water, may be subject to microbiological pollution. Enterococcus spp and E. coli are used as water quality indicators in the European Union (Directive 2006/7/EC) and the United States (2012 Recreational Water Quality Criteria RWQC published by US Environmental Protection Agency (EPA)). In this work, we propose a multiparametric detection of these 2 microorganisms in less than 3 hours after bathing water collection in accordance with EU and EPA regulations This rapid and quantitative analysis is based on RT-PCR specifically dedicated to viable bacteria.