[PRECELLYS] RNA extraction from regenerated limbs

Frog and newt are typical vertebrates famous for their regenerative abilities. To elucidate this molecular biological mechanism, we examine specific RNA expression levels on their regenerating tissues as daily work. To get reliable results from RNA expression assays, a quality/quantity of extracted RNAs is critical. In this report, we compare RNA yields by the Minilys bead beater against the BioMasherII disposable manual tissue grinder. The grinder is our former tool of homogenization.