It is important to study the quality of fish as food for the well-being of humans and animals. In this case, enzyme activity measurement allows evaluating potential fish muscle degradation, both pre-and postmortem. To evaluate the influence of different lengths of pre-slaughter crowding stress on fish fillet quality, total cathepsin B activity was measured in salmon muscle.

Cathepsins are lysosomal proteases that play a vital role in protein degradation, energy metabolism, and immune responses.

In this application note, discover how by using Bertin’s homogenizer, it was possible to successfully measure cathepsin activity and evaluate the influence of stress on fish quality.

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